Balleza, EnriqueKim, J. MarkCluzel, Philippe2018-06-272017Balleza, Enrique, J. Mark Kim, and Philippe Cluzel. 2017. “Systematic characterization of maturation time of fluorescent proteins in living cells.” Nature methods 15 (1): 47-51. doi:10.1038/nmeth.4509. http://dx.doi.org/10.1038/nmeth.4509.http://nrs.harvard.edu/urn-3:HUL.InstRepos:37160414Slow maturation time of fluorescent proteins limits accurate measurement of rapid gene expression dynamics and effectively reduces fluorescence signal in growing cells. We used high-precision time-lapse microscopy to characterize, at two different temperatures in E. coli, the maturation kinetics of 50 FPs that span the visible spectrum. We identified fast-maturing FPs that yield the highest signal-to-noise ratio and temporal resolution in individual growing cells.en-USJournal Article2018-06-2710.1038/nmeth.4509